Sumo protocols

  • 324 Pages
  • 4.28 MB
  • English

Humana Press , New York, NY
Statementedited by Helle D. Ulrich.
SeriesSpringer protocols, Methods in molecular biology -- 497
ContributionsUlrich, Helle D.
LC ClassificationsQP552.U24 S86 2009
The Physical Object
Paginationx, 324 p. :
ID Numbers
Open LibraryOL23677014M
ISBN 139781934115800
LC Control Number2008931179

Details Sumo protocols EPUB

"The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques Sumo protocols book.

In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation.

SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation Sumo protocols book the methods included in the book.

Description Sumo protocols FB2

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Abstract This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols. GOAT SUMO - Helmut Newton’s SUMO - David Bailey - David Hockney.

A Bigger Book - Annie Leibovitz. About this book This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation.

SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book.

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The book is. Introduction.

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SUMO is a member of the ubiquitin (Ub) and ubiquitin-like (Ubl) family. Post-translational attachment of SUMO to target proteins occurs through an enzymatic cascade analogous to the ubiquitin conjugation cascade (E1-E2-E3 enzymes), ultimately resulting in formation of an isopeptide bond between the Ub/Ubl C-terminal residue and substrate lysine residue (1,2).

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It is a craft of phenomenal. Called "One of the finest compact books on sumo today " by Sumo World, this pocket guide describes the history, rules, rituals and techniques of sumo. This sumo guide brings to life a cultural pastime that is much more than a sport with all the handy, up-to-date and authoritative you'll need to understand, appreciate and enjoy this traditional passion.

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My favorite takeaways from Skin Care Practices and Clinical Protocols book. SUMO Protocols (Methods in Molecular Biology) by Helle D. Ulrich (Editor) Hardcover, Pages, Published ISBN / ISBN / Post-translational protein modifications by members of the ubiquitin family are widely recognized as.

Books shelved as sumo: Sumo by Thien Pham, The Street of a Thousand Blossoms by Gail Tsukiyama, SumoKitty by David Biedrzycki, The Big Book of Sumo: Hist. Suggested Upper Merged Ontology (SUMO).

Contribute to ontologyportal/sumo development by creating an account on GitHub. However, the SUMO transgenic mice generated so far and the protocols developed for enriching SUMOylated proteins from tissue samples impressively illustrate the potential of this approach for future studies [33,].

It is, therefore, foreseeable that in the near future, additional SUMO transgenic mouse models will be developed. The sumo deadlift is an exercise becoming increasingly popular in the strength and conditioning environment, both for improving physical performance and as a potential rehabilitation tool.

SUMO Protocols. / Helle D. Ulrich. (Methods in Molecular Biology; Vol. Research output: Chapter in Book/Report/Conference proceeding › Chapter Gocke, CB & Yu, HIdentification of SUMO targets through in vitro expression cloning.

in HD Ulrich (ed.), SUMO Protocols. SUMO proteins are similar to ubiquitin in their folded structure but possess only about 20% homology to the amino acid sequence of ubiquitin.

The number of SUMO genes varies among eukaryotes. Organisms such as yeast, C. elegans, and Drosophila contain a single SUMO gene in their genome whereas plants and vertebrates possess several SUMO genes. In humans, there are four different SUMO genes.

Attachment of the small ubiquitin-like modifier (SUMO) to substrate proteins modulates their turnover, activity, or interaction partners. However, how this SUMO conjugation activity concentrates the proteins involved and the substrates into uncharacterized nuclear bodies (NBs) remains poorly understood.

Here, we characterized the requirements for SUMO NB formation and for their subsequent. Following cleavage of a C-terminal prosegment, the C-terminal glycine residue of SUMO is enzymatically attached to a lysine residue on a target protein.

In humans, SUMO is conjugated to a variety of molecules in the presence of the SAE1/UBA2 SUMO-activating (E1) enzyme and the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme.

The book includes the latest on Web protocols and new hardware, plus very timely information on how TCP/IP secures connectivity for blogging, vlogging, photoblogging, and social networking. This chapter will discuss various adaptations of the yeast two-hybrid method for analyzing protein interactions that can be used to identify small ubiquitin-related modifier (SUMO) interacting proteins and to determine the nature of the SUMO-protein interactions that occur.

Sumo (Japanese: 相撲, Hepburn: sumō, lit. "striking one another") is a form of competitive full-contact wrestling where a rikishi (wrestler) attempts to force his opponent out of a circular ring or into touching the ground with any body part other than the soles of his feet (usually by throwing, shoving or pushing him down).

Sumo originated in Japan, the only country where it is. Deduces both covalent sumoylation sites and non-covalent SUMO-interaction motifs (SIMs) in proteins. GPS-SUMO is a program based on a group-based prediction system (GPS) algorithm coupled to a particle swarm optimization approach.

The application can be set by using different prediction thresholds. Predictions with files with a size up to 2M have to be performed with the standalone version of.

A web server for sequence-based prediction of protein sumoylation sites. Users can enter an amino acid sequence or multiple protein sequences in the FASTA format, specify the methods (either random forest or support vector machine), and input the proper threshold for prediction of protein sumoylation site.

Bioinformatics Tools for Exploring the SUMO Gene Network. (source: Nielsen Book Data) Summary This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development.

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